We are pleased to announce that a pilot project has been awarded by the NIAID through the FluDyNeMo program.
Glycomic analysis of host response in influenza pathogenesis
Lara Mahal, New York University. New York, NY
Influenza infection is initiated in the upper airway through adhesion of viral hemagglutinin (HA) to specific glycan receptors found on the surface of the upper respiratory tract (Į2,6 linked sialic acids in humans and ferrets). Propagation of the virus requires cleavage from sialic acid containing receptors through expression of a sialidase (neuraminidase), the current target of anti-viral therapies. In addition, glycans play roles in host response and glycosylation patterns may be dynamic over the course of influenza infection, although there are few studies on this. We predict that specific glycans may be drivers of influenza severity.
We will use our lectin microarray technology to analyze samples generated in the FluDyNeMo study to identify specific glycan changes that map onto the pathology of influenza (i.e. the relationship between severity and age, levels of severity). Our data will be integrated with other datasets from the study to identify biomarkers and to examine the relationship between changes in the viral genome, host microbiome and host miRNA/transcriptome with glycosylation. The miRNA and transcriptome data will help us pinpoint underlying changes in glycosylation enzymes that lead to the observed glycosylation patterns. Our study would be one of the first on host-glycan response during an infectious process, providing insight into the relationship between glycans and disease outcome.