From the Mahal Lab

58. Agrawal, P; Fontanals-Cirera, B.; Sokolova, E.; Jacob, S.; Vaiana, C.A.; Argibay, D.; Davalos, V.; McDermott, M.; Nayak, S.; Darvishian, F.; Castillo, M.; Ueberheide, B.; Osman, I.; Fenyö, D.; Mahal, L.K.;; Hernando, E.‡  A Systems Biology Approach Identifies FUT8 as a Driver of Melanoma Metastasis. Cancer Cell 2017, 31, 804-819. doi:10.1016/j.ccell.2017.05.007. Co-corresponding authors.

57. Daley, D.; Mani, V.R; Mohan, N.; Akkad, N.; Ochi, A.; Lee, K.B.; Heindel, D.W., Zambrinis, C.O.; Werba, G.; Barrilla, R.M.; Torres-Hernandez, A.; Nayak, S.; Wang, D.; Hundeyin, M.; Ismail, K.; Diskin, B.; Aykut, B.; Rodriguez, R.; Chang, S.; Gardner, L.; Mahal, L.K.; Ueberheide, B.; Miller, G. Dectin-1 Activation on Macrophages by Galectin-9 Promotes Pancreatic Carcinoma and Peritumoral Immune-Tolerance. Nature Medicine 2017, 23, 556-567. doi: 10.1038/nm.4314.

56. Neelamegham, S.; Mahal, L.K. Multi-level regulation of cellular glycosylation: from genes to transcript to enzyme to structure. Curr. Opin. Struct. Biol. 2016, 40, 145-152.

55. Ribeiro, J.P.; Pau, W.K.; Pifferi, C.; Renaudet, O; Varrot, A; Mahal, L.K.; Imberty, A. Characterization of a high-affinity sialic acid specific CBM40 from Clostridium perfringens and engineering of a divalent form. Biochem. J. 2016, 473, 2109-18. doi: 10.1042/BCJ20160340. Co-corresponding authors.

54.Grant, O.C.; Tessier, M.B.; Meche, L.; Mahal, L.K.; Foley, B.L.; Woods, R.J. Combining 3D Structure with Glycan Array Data Provides Insight into the Origin of Glycan Specificity. Glycobiology, 2016, 26, 772-83. doi: 10.1093/glycob/cww020.

53.Hoashi, M.; Meche, L.; Mahal, L.K.; Bakacs, E.; Nardella, D.; Naftolin, F.; Bar-Yam, N.; Dominguez-Bello, M.G. Human Milk Bacterial and Glycosylation Patterns Differ by Delivery Mode. Reproductive Sciences, 2016, 23, 902-7. doi: 10.1177/1933719115623645.

52. Agre, P.; Bertozzi, C.; Bissell, M.; Campbell, K.; Cummings, R.; Desai, U.; Estes, M.; Flotte, T.; Fogleman, G.; Gage, F.; Ginsburg, D.; Gordon, J.; Hart, G.; Hascall, V.; Kiessling, L.; Kornfeld, S.; Lowe, J.; Magnani, J.; Mahal, L.K.; Medzhitov, R.; Roberts, R.; Sackstein, R.; Sarkar, R.; Schnaar, R.; Schwartz, N.; Varki, A.; Walt, D.; Weissman, I. Training the Next Generation of Biomedical Investigators in Glycoscience, J. Clin. Invest. 2016, 126, 405-408.

51. Vaiana, C.A.; Kurcon, T.; Mahal, L.K. MicroRNA-424 Predicts a Role for β-1,4 Branched Glycosylation in Cell Cycle Progression. J. Biol. Chem., 2016, 291, 1529-37. doi: 10.1074/jbc.M115.672220.

50. Kurcon, T.; Liu, Z.; Paradkar, A.V.; Vaiana, C.A.; Koppolu, S.; Agrawal, P.; Mahal, L.K. miRNA proxy approach reveals hidden functions of glycosylation. Proc. Natl. Acad. Sci., USA, 2015, 112, 7327-32. doi: 10.1073/pnas.1502076112.

49. Moncla, B.J.; Chappell, C.A.; Mahal, L.K.; Debo, B.M.; Meyn, L.A.; Hillier, S.L. Impact of bacterial vaginosis, as assessed by nugent criteria and hormonal status on glycosidases and lectin binding in cervicovaginal lavage samples. PLoS One, 2015, 10, e0127091. doi: 10.1371/journal.pone.0127091.

48. Wang, L.; Koppolu, S.; Chappell, C.; Moncla, B.J.; Hillier, S.L.; Mahal, L.K. Studying the effects of reproductive hormones and bacterial vaginosis on the glycome of lavage samples from the cervicovaginal cavity. PLoS One, 2015, 10, e0127021. doi: 10.1371/journal.pone.0127021.

47. Ng, S.; Lin, E; Kitov, P.I.; Tjhung, K.F.; Gerlits, O.O.; Deng, L.; Kasper, B.; Sood, A.; Paschal B.M.; Zhang, P.; Ling, C.C.; Klassen, J.S.; Noren, C.J.; Mahal, L.K., Woods, R.J.; Coates, L.; Derda, R. Genetically encoded fragment-based discovery of glycopeptide ligands for carbohydrate-binding proteins. J. Am. Chem. Soc., 2015, 137, 5248-51. doi: 10.1021/ja511237n.

46. Bonzi, J.; Bornet, O.; Betzi, S.; Kasper, B.; Mahal, L.K.; Mancini, S.; Schiff, C.; Sebban-Krauzer, C.; Guerlesquin, F.; Elantak, L. Binding of Galectin-1 to Pre-B Receptor Modulates Specific Galectin/Glycan Lattice Interactions Within the Bone Marrow Pre-BII Cell Niche. Nature Communications, 2015, 6, 6194. doi: 10.1038/ncomms7194.

45. Liang, Y.; Eng, W.S.; Colquhoun, D.R.; Dinglasan, R.R.; Graham, D.R.; Mahal, L.K. Complex N-linked Glycans Serve as a Determinant for Exosome/Microvesicle Cargo Recruitment. J. Biol. Chem., 2014, 289, 32526-37. doi: 10.1074/jbc.M114.606269.

44. Wang, L.; Cummings, R.D.; Smith, D.F.; Huflejt, M.; Campbell, C.T.; Gildersleeve, J.D.; Gerlach, J.Q.; Kilcoyne, M.; Joshi, L.; Serna, S.; Reichardt, N.-C.; Pera, N.P.; Pieters, R.; Eng, W.S.; Mahal, L.K. Cross-Platform Comparison of Glycan Microarray Formats. Glycobiology, 2014, 24, 507-517. Doi: 10.1093/glycob/cwu019.

43. Agrawal, P.; Kurcon, T.; Pilobello, K.T.; Rakus, J.F.; Koppolu, S.; Liu, Z.; Batista, B.S.; Eng, W.S., Hsu, K.-L.; Liang, Y.; Mahal, L.K. Mapping posttranscriptional regulation of the human glycome uncovers microRNA defining the glycocode. Proc. Natl. Acad. Sci., USA, 2014, 111, 4338-43. doi: 10.1073/pnas.1321524111.

42. Kasper, B.T.; Koppolu, S.; Mahal, L.K. Insights into MiRNA Regulation of the Human Glycome. Biochem. Biophys. Res. Commun. 2014, 445, 774-9. doi: 10.1016/j.bbrc.2014.01.034.

41. Ribeiro, J.P.; Mahal, L.K. Dot by dot: analyzing the glycome using lectin microarrays. Curr. Opin. Chem. Biol. 2013, 17, 827-31.

40. Pilobello, K.T; Agrawal, P.; Rouse, R.; Mahal, L.K. Advances in lectin microarray technology: Optimized protocols for piezoelectric print conditions. Curr. Prot. Chem. Biol. 2013, 5, 1-23.

39. Bird-Lieberman, E.L.; Neves, A.A.; Lao-Sirieix, P.; O’Donovan, M.; Novelli, M.; Lovat, L.B.; Eng, W.S.; Mahal, L.K.; Brindle, K.M.; Fitzgerald, R.C. Molecular imaging using fluorescent lectins permits rapid endoscopic identification of dysplasia in Barrett’s esophagus. Nat. Medicine    2012, 18, 315-21.

38. Reuel, N.F.; Ahn, J.-H.; Kim, J.-H.; Zhang, J.; Boghossian, A.A.; Mahal, L.K.; Strano, M.S. Transduction of Glycan␣Lectin Binding Using Near-Infrared Fluorescent Single-Walled Carbon Nanotubes for Glycan Profiling. J. Am. Chem. Soc., 2011, 133, 17923-33.

37. Batista, B.S.; Eng, W.S.; Pilobello, K.T.; Hendricks-Muñoz, K.; Mahal, L.K. Identification of a     Conserved Glycan Signature for Microvesicles. J. Proteome Res. 2011, 10, 4624-33.

36. Gaziel-Sovran, A; Segura, M.F.; Di Micco, R; Collins, M.K.; Hanniford, D.; Vega-Saenz de Miera, E.; Rakus, J.F.; Dankert, J.F.; Shang, S.; Kerbel, R.S.; Bhardwaju, N.; Yongzhao, S.;    Darvishan, F.; Zavadil, J.; Erlebacher, A.; Mahal, L.K.; Osman, I.; Hernando, E. MiR-30b/30d      regulation of GalNAc transferases enhances invasion and immunosuppression during metastasis. Cancer Cell 2011, 20, 104-18.

35. Propheter, D.C.; Hsu, K.-L.; Mahal, L.K. Recombinant lectin microarrays for glycomic analysis. Methods Mol. Biol. 2011, 723, 67-77.

34. Rakus, J.F.; Mahal, L.K. New Technologies for Glycomic Analysis: Toward a Systematic  Understanding of the Glycome. Ann. Rev. Anal. Chem. 2011, 4, 367-92.

33. Propheter, D.C.; Mahal, L.K. Orientation of GST-tagged lectins via in situ surface modification to create an expanded lectin microarray for glycomic analysis. Mol. Biosystems 2011, 7, 2114-7.

32. Krishnamoorthy, L.K.; Mahal, L.K., Lectin Microarrays: Simple Tools for the Analysis of  Complex Glycans, chapter in Functional and Structural Proteomics of Glycoproteins, eds. Owens, R.J; Nettleship, J.E., 2011, Springer Verlag.

31. Carillo, L.D.; Froemming, J.A.; Mahal, L.K., Targeted in Vivo O-GlcNAc Sensor Reveals Discrete Compartment-specific Dynamics During Signal Transduction. J. Biol. Chem. 2011,   286, 6650-6658.

30. Hsu, KL; Pilobello, K.; Krishnamoorthy, L; Mahal, L.K., Ratiometric lectin microarray analysis of the mammalian cell surface glycome. Methods Mol. Biol. 2011, 671, 117-31.

29. Propheter, D.C.; Hsu, K.-L.; Mahal, L.K. Fabrication of an Oriented Lectin Microarray,  ChemBioChem, 2010, 11, 1203-1207.

28. Krishnamoorthy L.; Mahal L.K. Glycomic analysis: an array of technologies. ACS Chem Biol. 2009, 4, 715-732.

27. Lebrilla C.B.; Mahal L.K. Post-translation modifications. Curr Opin Chem Biol. 2009 13, 373-374.

26. Hsu K.L.; Mahal L.K. Sweet tasting chips: microarray-based analysis of glycans. Curr Opin Chem Biol. 2009. 13, 427-432.

25. Krishnamoorthy, L.; Bess, J.W.; Preston, A.B.; Nagashima, K.; Mahal, L.K. HIV-1 and microvesicles from T cells share a common glycome, arguing for a common origin, Nature Chem. Biol. 2009 5, 244-250.

24. Hsu, K.-L.; Gildersleeve, J.C.; Mahal, L.K. A simple strategy for the creation of a recombinant lectin microarray, Mol. BioSystems, 2008, 4, 654-662.

23. Mahal, L.K. Glycomics: Towards Bioinformatic Approaches to Understanding Glycosylation,  Anti-Cancer Agents Med. Chem. 2008, 8, 37-51.

22. Pilobello, K.T.; Mahal, L.K. Lectin Microarrays for Glycoprotein Analysis, Methods Mol. Biol., 2008, 385, 193-203.

21. Pilobello, K.T.; Slawek, D.; Mahal, L.K. A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome, Proc. Natl. Acad. Sci., USA, 2007, 104, 10534-10539.

20. Pilobello, K.T.; Mahal, L.K. Deciphering the glycocode: the complexity and analytical challenge of glycomics, Curr. Opin. Chem. Biol., 2007 11, 300-305.

19. Carrillo, L.D.; Krishnamoorthy, L.; Mahal, L.K. A Cellular FRET Sensor for β-O-GlcNAc, a Dynamic Carbohydrate Modification Involved in Signaling, J. Am. Chem. Soc., 2006, 128, 14768-14769.

18. Hsu, K.-L.; Mahal, L. K. Profiling the sweet structures of the bacterial glycome. Nature Protocols, 2006, 1, 543-549.

17. Sanki, A.; Mahal, L.K. A One-Step Synthesis of Azide-Tagged Carbohydrates: Versatile Intermediates for Glycotechnology. Synlett 2006, 3, 455-459.

16.  Hsu, K.-L.; Pilobello, K.T; Mahal, L.K. Analyzing the dynamic bacterial glycome with a lectin microarray approach. Nature Chem. Biol. 2006, 2, 153-157.

15. Pilobello, K.T.; Krishnamoorthy, L.; Slawek, D.; Mahal, L.K. Development of a Lectin Microarray for the Rapid Analysis of Protein Glycopatterns. ChemBioChem 2005, 6, 985-989.

14.  Mahal, L.K. Catching Bacteria with Sugar. Chem. & Biol., 2004, 11, 1602-1604.


From Postdoctoral Work (with Prof. James E. Rothman, Sloan-Kettering Institute)

13. Melia, T.J.; Weber, T.; McNew, J.A.; Fisher, L.E.; Johnston, R.J.; Parlati, F.; Mahal, L.K.; Söllner, T.H.; Rothman, J. E. Regulation of Membrane Fusion by Conformational Switching of  the Membrane-Proximal Coil of the t-SNARE During Zippering of SNAREpins. J. Cell Biol. 2002, 158, 929-9.

12. Mahal, L.K.; Sequeira, S.M.; Gureasko, J.M.; Söllner, T.H. Calcium-Independent Stimulation of Membrane Fusion and SNAREpin Formation by Synaptotagmin I. J. Cell Biol. 2002, 158, 273-282.


From Graduate Work (with Prof. Carolyn Bertozzi, U.C. Berkeley)

11. Charter, N.W.; Mahal, L.K.; Koshland, D.E., Jr.; Bertozzi, C.R. Differential Effects of Unnatural Sialic Acids on the Polysialylation of Neuronal Cell Adhesion Molecule and Neuronal Behaviour. J. Biol. Chem. 2002, 277, 9255-9261.

10. Mahal, L.K.; Charter, N.W.; Angata, K.; Fukuda, M.; Koshland, D.E., Jr.; Bertozzi, C.R. A Small Molecule Modulator of Poly-a-2,8-Sialic Acid Expression on Neurons and Tumor Cells. Science 2001, 294, 380-381.

9. Groves, J.T.; Mahal, L.K.; Bertozzi, C.R. Control of Cell Adhesion and Growth with Micropatterned Supported Lipid Membranes. Langmuir 2001, 17, 5129-5233.

8. Jacobs, C.L.; Yarema, K.J.; Mahal, L.K.; Nauman, D.A.; Charter, N.W.; Bertozzi, C.R. Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Biosynthesis. Methods Enzymol. 2000, 327, 260-275.

7. Charter, N.W.*; Mahal, L.K.*; Koshland, D.E., Jr.; Bertozzi, C.R. Biosynthetic Incorporation of    Unnatural Sialic Acids into Polysialic Acid on Neural Cells. Glycobiology 2000, 10, 1049-1056. * co-first author.

6. Lee, J.H.; Baker, T.F.; Mahal, L.K.; Zabner, J.; Bertozzi, C.R.; Weimer, D. F.; Welsh, M. J.  Engineering Novel Cell Surface Receptors for Virus-Mediated Gene Transfer. J. Biol. Chem. 1999, 274, 21878-84.

5. Yarema, K.J.; Mahal, L.K.; Bruehl, R.E.; Bertozzi, C.R. Metabolic Delivery of Ketone Groups     to Sialic Acid Residues. Application To Cell Surface Glycoform Engineering. J. Biol. Chem. 1998, 273, 31168-79.

4. Mahal, L.K.; Bertozzi, C.R. Engineered Cell surfaces: fertile ground for molecular landscaping Chemistry & Biology 1997, 4, 415-22.

3. Mahal, L.K.; Yarema, K.J.; Bertozzi, C.R. Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis. Science 1997, 276, 1125-1128.


From Undergraduate Work (with Prof. Rebecca Braslau, U.C. Santa Cruz)

2.  Braslau, R.; Burrill, L.C.; Siano, M.; Naik, N.; Howden, R.K.; Mahal, L.K. Low-Temperature Preparations of Unimolecular Nitroxide Initiators for “Living” Free Radical Polymerizations, Macromolecules 1997, 30, 6445-6450.

1. Braslau, R.; Burrill, L.C.;  Mahal, L.K.; Wedeking, T.  A Totally Radical Approach to the Control of Stereochemistry: Coupling of Prochiral Radicals with Chiral Nitroxyl Radicals. Angewandte Chemie 1997, 36, 237-238.