NMR Experiment Guide

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  8. Pseudo 2D Sequence (zg2d)

Pseudo 2D Sequence (zg2d)

  • zg2d is a 2D sequence designed to acquire a series of 1D spectra with a defined delay prior to the acquisition of each slice.  The data is stored in a 2D (ser) file.
  • Sequence Setup
    • In the ACQUPARS tab, set PULPROG to zg2d 
    • Press to set the parmode (data dimensionality) from 1D to 2D
    • Type td in the command line and set the F1 dimension to the number of 1D spectra you want to acquire
    • Type d20 in the command line and set the delay between the start of each experiment.  In the sequence, DELTA is the amount of time after the total acquisition time of a 1D experiment is taken into account to ensure an accurate timing between experiments.
    • Use the experiment time command, expt, to help adjust td (F1) and d20 to to maximize your reserved time slot  
    • Acquire the data, zg 
Sample Spectrum
    • When using the zg2d sequence you are left with a ser file. 
    • For quick viewing:
      • Process the F2 dimension using the commands xf2 (Fourier Transform), apk2d (automatic phase correction), and abs2 (automatic baseline correction in the F2 dimension).
      • Press the  icon to view an oblique rendering.  and   icons can be used to change the view.
      • Press  to enter the contour mode
        • Decrease the intensity of the contours as low as possible
        • Press to get to the multidisplay mode
        • Press  to scan rows of the contour plot
        • Use your mouse cursor to move along the F1 dimension to view the 1D spectrum of a specific row
    • Alternatively, the command splitser will separate the rows of the 2D file and extract into 1D experiment files which can be processed individually or as a batch using the multicmd command.
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