Phase sensitive 2D homonuclear NOE experiment using gradient pulses for selection and purge pulses
Correlates chemical shifts of spins that have a mutual J-coupling that are 2 to 4 bonds apart.
Gradients eliminates the need for phase cycling and helps reduce T1 noise and other artifacts
Scans per Slice
(ns)
Dummy Scans
(ds)
Recycle
Delay
(d1)
FID Size
(TD, F2)
# Slices
(TD, F1)
Spectral
Width
(sw,ppm)
Spectral
Center
(o1p, ppm)
2 x ns (16)
16
2
2048
256
10
4.7
Experiment Setup
Use getprosol or prosol button to read in proper pulse parameters
Set d8 is the mixing time; must be long enough for NOE build up:
Small molecules: d8 = T1
Large molecules: d8 = 0.5 T1
Default d8 = 300 ms
Processing Notes
Fourier transform with the command xfb
Manually phase F2 and F1 dimensions
Use commands abs2 and abs1 for baseline correction
For improved resolution, can try applying linear prediction in the F1 dimension. In the ProcPars tab set the following parameters:
ME_mod = LPfr
NCOEF = set to 3 times the number of cross peaks in your spectrum. For NOESY spectra, count symmetric peaks as two. Values that are incorrect by more than a factor of two can degrade the resolution.
