DEPT – Shared Instrumentation Facility

Parameter Set		C13DEPT135		Pulse Sequence		dept135
Experiment Description		Signal enhancement by polarization transfer from ¹H to ¹³C with spectral editing DEPT135: θ = 135, CH and CH₃ positively phased, CH₂ negatively phased. Other options: DEPT45: θ = 45, all protonated carbons are shown DEPT90: θ = 90, primarily methane carbons, possible small breakthroughs of some other signals
Scans (ns)	Dummy Scans (ds)	Recycle Delay (d1)	1D Loop (TD0)	FID Size (TD, F2)	Spectral Width (sw,ppm)	Spectral Center (o1p, ppm)
4 x ns (64)	8	2	4	64k	240	105
Experiment Setup		Use getprosol or prosol button to read in proper pulse parameters Set cnst2 = ¹J_CH coupling constant: default cnst2 = 140 Hz If poor signal cancellation is possible Use at least 8 dummy scans (ds) Keep temperature constant, turn on VT Use proper pulse widths, always update prosol pars Ensure the sample is locked
Processing Notes		Phase so that CH/CH₃ peaks are positive and C/CH₂ are negative
Sample Spectrum		Enlarge Spectrum from: https://www.nanalysis.com