The Varian Cary 100 Bio UV-vis spectrophotometer is a very versatile instrument for life science applications. The bio-analysis package that is used with this system includes a concentration module for concentration measurement of proteins, kinetics/enzyme kinetics module for determining reaction rates and enzyme activity, and an RNA/DNA module to determine the purity and type of nucleic acid present. With the temperature controller accessory, and the thermal application, denaturation/renaturation measurements and DNA thermal melting experiments are made possible.
The Varian Cary 100 Bio also has a temperature controller accessory that connects to the electrothermal 6×6 Peltier multicell holder. Temperature ramp rates as slow as 0.06 °C/min can be used for high resolution DNA thermal melting experiments. Users can define up to 20 different temperature ramp rates and directions in the same data file. The Thermal application software can calculate the thermal melt temperature. Calculations of thermodynamic parameters for DS, DH, K at Tm , DG and K at user defined temperatures are also available.
Key Specifications:
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- Accuracy: ±2 nm
- Wavelengths: 190 to 900 nm
- Light Source: Tungsten Halogen
- Temperature Range: -10 to 100 °C
- Variable slit widths for optimum control over spectral resolution.
- Working range past 3.5 absorbance units eliminates sample dilution.
- Phase-locked wavelength prevent peak shifts and suppression at high scan speeds.
The Cary 100 Bio UV-Visible Spectrophotometer was acquired through the support of New York University.